scanpy合并多个adata数据集和根据条件进行随机抽取细胞

import pandas as pd ; import numpy as np ; from scipy import sparse
import scanpy as sc
import anndata as ad
import os

加载datafram格式的rna_counts数据集构建AnnData

X = pd.read_csv(r'./1.rnacounts.tsv', sep = '\t').transpose()
X = sparse.csr_matrix(X)
ad_1 = ad.AnnData(X = X.values, obs = pd.DataFrame(index = X.index), var = pd.DataFrame(index = X.columns))
ad_1.var_names_make_unique()
ad_1.obs_names_make_unique()
X = pd.read_csv(r'./2.rnacounts.tsv', sep = '\t').transpose()
X = sparse.csr_matrix(X)
ad_2 = ad.AnnData(X = X.values, obs = pd.DataFrame(index = X.index), var = pd.DataFrame(index = X.columns))
ad_2.var_names_make_unique()
ad_2.obs_names_make_unique()
X = pd.read_csv(r'./3.rnacounts.tsv', sep = '\t').transpose()
X = sparse.csr_matrix(X)
ad_3 = ad.AnnData(X = X.v