下载centos7 镜像并制作U盘启动盘
从清华镜像源进行下载
wget https://mirrors.tuna.tsinghua.edu.cn/centos/7.7.1908/isos/x86_64/CentOS-7-x86_64-DVD-1908.iso
设置U盘为第一启动项
开机F9可以进入BIOS设置 {{uploading-image-412605.png(uploading...)}}
系统环境搭建
系统版本如下: CentOS Linux release 7.7.1908 (Core)
安装完成后不能进入centos界面系统,使用如下命令进入命令行操作 ctrl alt F2 进入命令行,输入用户名和密码
打开网络,并设置自动连接
cd /etc/sysconfig/network-scripts
ifup ifcfg-enp4s0f2
安装R 3.6.1
yum install R
R包的安装
need_packages=c("reshape2","RColorBrewer","ggplot2","plyr","grid","Mfuzz","WGCNA","pheatmap")
install.packages(need_packages)
BGI need R package
if (!requireNamespace("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install(version = "3.10")
BiocManager::install("AnnotationDbi","EBSeq","NOISeq","DEGseq","clusterProfiler")
BiocManager::install("topGO")
BiocManager::install("Rgraphviz")
BiocManager::install(c("impute","preprocessCore")) #WGCNA
DESeq2安装报错
jpeglib.h: No such file or directory
安装如下系统依赖
yum install -y libjpeg-devel
perl 模块的安装
手动安装GD模块,安装完成后会报错
wget http://www.cpan.org/authors/id/L/LD/LDS/GD-1.16.tar.gz
cd GD-1.16
perl Makefile.PL
make
make install
安装gd依赖,发现可能还是存在一些问题,考虑使用cpan进行安装
yum install -y perl-GD
yum install -y gd-devel
最后使用cpan安装
cpan install YAML
cpan install Module:Build
cpan install Excel::Writer::XLSX
安装module
参考module安装日志
conda 安装并设置镜像源
channels:
- https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/conda-forge/
- https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/free/
- defaults
- r
show_channel_urls: true
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/free/
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/main/
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/conda-forge/
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/bioconda/
/home/yueyao/bio/tools/miniconda/4.5.1/bin/conda create -p /home/yueyao/bio/tools/snakemake/3.13.3 snakemake
安装texlive
参考texlive安装日志
安装fastqc
wget fastqc_v0.11.8.zip
unzip fastqc_v0.11.8.zip
mkdir -p /usr/local/Modules/modulefiles/fastqc/0.11.8